Downstream Processing

Overview

• This intensive Downstream Processing course at Delft University of Technology (TU Delft) consists of theoretical and applied lectures, exercises, case studies and computer simulations. The course will be given in English. A pocket calculator is required.

• The course presents the state of the art in downstream processing of biotechnological products. It provides knowledge of different techniques for solid-liquid separation, product release, refolding, concentration and purification of precious biotechnology products with a focus on the total integrated process. 

• An international group of experts from both industry and academia will lecture to assure an optimal balance of practical knowledge and theoretical insight, which subsequently will be trained by exercises, into the quantitative engineering aspects of bioseparations. 
• At the end of the course, the participants should be able to estimate main equipment dimensions, know qualitative constraints to the integrated bioprocess and have a quantitative insight in process streams, structure and economics.

Learning outcomes

• The course starts with an overview of possibilities and problems typically associated with the recovery of bioproducts. Physico-chemical characteristics of the products, as well as the fermentation broth with its multiple contaminants, are discussed in relation to possibilities for selective separation. Using this information, the general structure of large scale industrial processes will be outlined.
• The thermodynamical basics and biochemical engineering principles will be introduced and developed during the course related to bio-separations. Special attention will be paid to the unit operations typically used in this field of industry. 
• Firstly, various solid-liquid separation techniques and cell disruption will be treated. Particular consideration will be given to the application of major concentration techniques such as extraction, adsorption and membrane separation. 
• Furthermore, the scientific and industrial aspects of purification by means of precipitation and chromatography will be discussed. In addition, the release of intracellular products as well as protein folding in industrial protein processes will be discussed.
• Process integration is the binding element in the course. During the course, a case study on the design of an integrated purification process for a recombinant protein will offer the participants the opportunity to practice on the individual unit operations as well as on the integrated process. Finally, possibilities of computer-aided, rational design of integrated separation processes will be demonstrated.

• This Advanced Course is aimed at professionals (MSc or PhD level is required) in (bio)chemical engineering, or in microbiology or biochemistry with a basic knowledge in chemical engineering. 
• The course is primarily aimed at those already employed in industry and who are interested in the separation of biotechnological products. In addition, this Advanced Course is an option in the two-year postgraduate programs of Delft University of Technology.